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Elements and Performance Criteria

1. Maintain records
   • Maintain records of batches of media
   • Complete records of tissue cultures so that they are retrievable, legible and accurate
   • Complete records in accordance with information management, records, quality system and legal requirements
2. Work safely according to the legal and regulatory framework
3. Identify hazards and workplace controls associated with the sample, preparation methods, reagents and equipment
4. Select, fit and use personal protective equipment (PPE)
5. Address hazards and incidents as they arise
6. Maintain a chain of custody, traceable to the worker, for all cells and tissues
7. Prepare and test cell and tissue culture media
   • Select and confirm media specifications and processes/methods
8. Prepare culture media to suit the application
9. Sterilise culture media and check for sterility
10. Perform quality control checks to ensure that culture media is fit for purpose
11. Store culture media in accordance with specifications
12. Prepare tissue or cell cultures
    • Select tissue/cell sample to optimise growth and prepare it for culture
13. Add specified growth agents and/or nutrients
14. Inoculate culture medium using aseptic techniques
15. Monitor tissue or cell culture
    • Incubate culture in specified conditions
16. Monitor growth of culture and record appearance and characteristics
17. Report presence or absence of contamination
18. Subculture the culture to continue the cell line
19. Dispose of biohazardous and other laboratory waste safely
    • Maintain records of batches of media
20. Complete records of tissue cultures so that they are retrievable, legible and accurate
21. Complete records in accordance with information management, records, quality system and legal requirements